inhibitors recombinant murine fgf8  (R&D Systems)

Journal: iScience

Article Title: Cell density couples tissue mechanics to control the elongation speed of the body axis

doi: 10.1016/j.isci.2024.110968

Figure Lengend Snippet: Integration of FGF signaling and elongation speed response (A) Representative confocal images of control embryos and embryos treated with the FGF inhibitor PD173074 for 30 min. Hoechst signal is shown on the left, the pMAPK signal is shown in the middle, Hoechst signal with PSM shape drawn for intensity analysis (using Fiji) is shown on the right. Regularly appearing, blurry dark horizontal lines are artifacts of the stitching of tiled confocal image edges. Scale bar: 100μm. (B) Average fluorescence intensity (normalized to the average intensity of the most posterior point +100μm on either side, bars indicate the mean, same in panel C) of pMAPK for controls (DMSO) and treated with PD173074 for 30 min and 1h. n = 15 (8 embryos) for DMSO Controls, n = 8 (4 embryos) for 30 min PD173074, n = 7 (4 embryos) for 1h PD173074 (∗∗ p = 0.0089, 30 min: n.s. p = 0.058, 1h: n.s. p = 0.95, paired t-test applied to samples for which there are datapoints at the two ends as not all pPSM images reach 600μm, same in panel C, n = 6 for 30 min PD173074). (C) Average fluorescence intensity of pMAPK for controls, after pulling and after compression. n = 20 (10 embryos) for controls, n = 9 for compressed (5 embryos), n = 10 for pulled (5 embryos) (∗∗∗p = 3e-4, ∗∗ p = 0.0017, n.s. p = 0.11, paired t-tests, n = 7 for compressed). (D) Elongation speed dynamics following pulling + FGF8 addition. n = 5 for control and pulled, n = 4 for +FGF8 and n = 6 for pulled + FGF8 (0-1h: ∗∗p = 3e-3, n.s. p = 0.913; 1-2h: ∗p = 7e-3, n.s. p = 0.431; 2 tailed t-tests). Standard boxplots with quartiles, SD error bars are used. (E) Average elongation speed over 4 h after compression + FGF inhibition. n = 12 for control (DMSO), n = 10 for FGF inhibitor (PD173064), n = 6 for compressed DMSO and n = 7 for PD173074 (∗ p = 0.011, ∗∗p = 4e-3, 2 tailed t-tests). Standard boxplots with quartiles, SD error bars are used.

Article Snippet: FGF8 recombinant mouse protein , R&D systems , 424-FC-025/CF.

Techniques: Control, Fluorescence, Inhibition

Journal: iScience

Article Title: Cell density couples tissue mechanics to control the elongation speed of the body axis

doi: 10.1016/j.isci.2024.110968

Figure Lengend Snippet:

Article Snippet: FGF8 recombinant mouse protein , R&D systems , 424-FC-025/CF.

Techniques: Recombinant, Software